詹璐,闫桂玲,胡薇

• 1:海军军医大学第一附属医院(上海长海医院)甲乳外科
• 2:东部战区海军医院普外科

摘要(Abstract)：

目的建立亚临床甲状腺功能亢进和减退2种不同甲状腺功能状态的小鼠模型,并明确亚临床甲状腺功能水平不同对小鼠Morris水迷宫实验中学习记忆行为的影响。方法选取30只Balb/C雄性小鼠,周龄5～6周,适应性饲养1周后,随机分为亚甲亢组、亚甲减组和对照组,每组10只。经高、低剂量摸索后确定造模用药剂量。观察3组小鼠对声、光等外界刺激的反应。3周后行Morris水迷宫实验,对比分析3组小鼠行为学差异。同时采集血清标本验证是否造模成功。结果亚甲亢组小鼠每日给予腹腔注射优甲乐25μg/100 g鼠重,亚甲减组每日给予小鼠腹腔注射甲巯咪唑(MMI)1.5 mg/100g鼠重,对照组常规喂养。亚甲亢组促甲状腺激素(TSH)为(0.27±0.09)mU/L,亚甲减组TSH为(3.15±0.68)mU/L,对照组TSH为(1.01±0.16)mU/L,3组差异有统计学意义(P<0.01),三碘甲状腺原氨酸(T3)、甲状腺素(T4)差异无统计学意义(P>0.05)。3组小鼠天冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)水平差异无统计学意义(P>0.05)。定位航行实验,与对照组相比,亚甲亢组每日逃避潜伏期明显缩短(P<0.05),分别为(68±29)s、(51±36)s、(41±39)s、(38±41)s,而亚甲减组差异无统计学意义(P>0.05)。空间探索实验,亚甲亢组目标象限路程比为(29±16)%,亚甲减组为(14±11)%,对照组为(15±11)%,亚甲亢组目标象限时间比为(33±20)%,亚甲减组为(15±14)%,对照组为(17±12)%,亚甲亢组穿台次数为2.0(1.75,3.0)次,亚甲减组为1.0(0,1.0)次,对照组为1.0(0.75,1.0)次。亚甲亢组3组指标均显著高于对照组(P<0.05),而亚甲减组差异无统计学意义(P>0.05)。3组平均速度差异无统计学意义(P>0.05)。结论运用优甲乐和甲巯咪唑腹腔注射的方法可成功建立亚甲亢和亚甲减小鼠动物模型。通过Morris水迷宫实验发现亚甲亢组小鼠的空间学习、记忆能力比对照组小鼠增强,而亚甲减组小鼠无明显差异。

关键词(KeyWords)： 认知;小鼠;亚临床甲状腺功能异常;Morris水迷宫实验

Abstract:

Keywords:

基金项目(Foundation): 上海长海医院院级科研项目(军事医学专项)(2018JS011);上海长海医院教学研究与改革项目(CHJG2019018)

作者(Author): 詹璐,闫桂玲,胡薇

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